Project Title: The isolation and validation of miRNA/mRNA complexes to identify
genes and pathways targeted by tumor suppressor miRNAs in prostate cancer cells
Funding: ITT Istituto Toscano Tumori
Partners: CNR-IFC (Pisa), CNR-IIT (Pisa).
Project Coordinator: Dr. Giuseppe Rainaldi (IFC-CNR)
Duration: 3 years (2013-2015).
Project value: 440,000 Euro
AIM OF THE PROJECT:
The challenge of the project is to discover through which mechanisms
two tumor suppressor miRNAs control cell proliferation and survival pathways.
As a reliable identification of miRNA targets using the currently available targets
prediction tools (algorithms) has proven to be quite difficult due to the high rates
of false positive, we propose to use a new and efficacious approach based on affinity
purification of miRNA/mRNA targets complexes in living cells.
The goal of the project is the experimental identification of Tumor Sopressors (TS) miRNA
targets in prostate tumor cells and this should expand the current knowledge
on the possible use of TS miRNAs as anticancer drugs.
Last update: November 4th, 2014
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